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KMID : 0613820220320030222
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Journal of Life Science
2022 Volume.32 No. 3 p.222 ~ p.231
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The Effects on Melanogenesis in B16F10 Melanoma Cells and the Anti- inflammatory Activities of an Ethyl Acetate Fraction from Glechoma hederacea var. longituba
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Yeom Hyeon-Ji
Oh Min-Jeong
Chae Jung-Woo
Lee Jin-Young
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Abstract
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This study aimed to confirm the possibility of being used as a cosmetic material material through the verification of the whitening and anti-inflammatory activities of an ethyl acetate fraction from Glechoma hederacea var. longituba (GHEA). The observed electron donating and ABTS+ radical scavenging abilities of GHEA were 89.6% and 88.7%, respectively, at 1,000 μg/ml concentration, with a tyrosinase inhibitory effect of 22.3% at the same concentration. For cell viability, a rate of 80% or more was observed in all concentrations that treated GHEA on melanoma and macrophage cells. Protein and mRNA expression inhibition was measured by Western blot and RT-PCR for 25, 50, and 100 μg/ ml concentrations, and it was confirmed that expression decreases in a concentration-dependent manner as GHEA concentration increases. The inhibition of the whitening-related factors MITF and TRP-2 were superior following GHEA treatment than those of the control group treated with kojic acid of 100 μg/ml concentration. For tyrosinase, the lowest mRNA expression rate was 29.1% at 100 μg/ml which confirmed excellent inhibition. In analyzing the effects of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α on protein and mRNA expression, IL-6 and TNF-α showed high protein and mRNA inhibition compared to a vitamin C control group. Based on these experimental results, GHEA could be applied as a natural cosmetic material.
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KEYWORD
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Anti-inflammatory, cytokine, Glechoma hederacea var. longituba, IL-6, whitening
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